Scientific Program

Conference Series Ltd invites all the participants across the globe to attend 14th Annual Conference on Stem Cell and Biomaterials Paris, France.

Day :

  • Regeneration & Therapeutics
Location: Paris, France
Speaker
Biography:

 
Cristina Grange has graduated in Medical Biotechnology in 2004 at the University of Turin (110/110 cum laude). She has done her phD in Medical Pathophysiology earning doctorate in 2007.
She is the author of 60 scientific publications in international journals (h-index is 27, https://orcid.org/0000-0002-6960-5476). She is guest editor for the special issue “Therapeutic Applications of Extracellular Vesicles” in Cells journal, in 2019; editor of Stem cells international, Annals of Stem Cells and Regenerative Medicine and  Scientific Journal of Stem Cell Research. She is member of International Society for Extracellular Vesicles
 

Abstract:

Extracellular Vesicles (EVs) released by stem cells carry transcriptional regulators and secreted RNAs and may transfer these encapsulated molecules to target cells, inducing phenotypic changes. It has been shown that EVs derived from stem cells, and in particular those derived from mesenchymal stromal cells (MSC), may reprogram injured cells and activate regenerative processes resulting  in repair of acute tissue injury. We herein investigate the potential therapeutic effect of EVs, shed by human bone marrow MSCs and by human liver stem-like cells (HLSCs), on the progression and reversion of fibrosis in two mouse models, diabetic nephropathy model, induced by streptozotocin and aristolochic acid-induced kidney fibrosis.
The stem cell-derived EV treatment, but not the fibroblast-EV treatment that was used as a control, significantly ameliorated renal functional parameters, such as albumin/creatinine excretion, plasma creatinine and blood urea nitrogen, which are altered in diabetic mice. Moreover, the renal fibrosis that develops during diabetic nephropathy progression was significantly inhibited in stem cell EV-treated quantified by histology and molecular analysis. A correlation between the down regulation of several pro-fibrotic genes, such as collagen, alpha-sma and TGF-beta in renal tissues, and the anti-fibrotic effect of HLSC and MSC EVs was found. A comparative analysis of HLSC and MSC EV miRNA content highlighted some common and some specific patterns of miRNAs that target predicted pro-fibrotic genes. 
 

  • Genomics
Location: Paris, France
Biography:

Shalini TV has completed MD in Ayurveda at the age of 27 years from Rajiv Gandhi University of Health Sciences, Karnataka, India. She is currently working as Research Associate in Ramaiah Indic Specialty Ayurveda, an Ayurveda treatment centre and Research Organisation. She has published around 6 papers in reputed Journals. She is also a part time practitioner of Ayurveda.

Abstract:

Prakriti (body –mind constitution of an individual) is a conventional, customized and unique the understanding of which is essential for the personalized medicine described in Ayurveda, Indian System of Medicine. Based on the Doshas( functional, bio humoral unit in the body) individuals are categorized into three major prakriti- Vata, Pitta and Kapha. The human gut micro biome hosts plenty of highly diverse and metabolically active microorganisms, mainly dominated by the bacteria, which are known to influence the physiology of an individual. Few researches have shown the correlation between the prakriti and the biochemical parameters. In this study, an attempt was made to explore any correlation between the prakriti (phenotype of an individual) with the Genetic makeup of the gut micro biome in healthy individuals.

  • Biodegradable Biomaterials
Location: Paris, France
Speaker
Biography:

Orly H. has completed her Ph.D. in Material Engineering from Ben Gurion University of the Negev, Israel, on August 2016. She is a lecturer at the Department of Mechanical Engineering, SCE- Shamoon College of Engineering, Israel. In addition, she is an academic coordinator of manufacturing technologies and material science courses and laboratories. She is a specialist in biodegradable magnesium implants, processing, including additive manufacturing, and physical metallurgy of alloys and environmental behavior of metals.
She has published 6 papers in reputed journals and has been serving as a reviewer for MDPI Journals.
 

Abstract:

Mg alloys are considered as potential structural materials for biodegradable implants mainly due to their excellent biocompatibility, degradation behavior in in vivo conditions and adequate mechanical properties. However, their accelerated corrosion rate in physiological environments may lead to premature loss of mechanical integrity and cytotoxic effects. Here we characterized the corrosion behavior and mechanical properties of a novel magnesium-zinc alloy, Mg-5%Zn-0.13%Y-0.35%Zr with up to 3% Nd additions following a homogenizing treatment and extrusion process, with regards to serving as a biodegradable implant. 
The microstructural characteristics were examined by optical microscopy, scanning electron microscopy (SEM), and X-ray diffraction analysis. The corrosion performance examination was carried out under in in vitro conditions, including immersion testing, electrochemical analysis, and stress corrosion cracking (SCC) assessments in terms of slow strain rate testing (SSRT), all in PBS solution. The mechanical evaluations included hardness and tensile examinations. 
The obtained results clearly demonstrated an optimal combination of strength and ductility for the new alloy at 2% Nd concentration. This was attributed to an optimal concentration of the secondary phase, W-phase (Mg3(Nd,Y)2Zn3), generated at grain boundaries. 
The addition of different concentrations of Nd to the base alloy, resulted in minor effect on the corrosion resistance, nevertheless, the calculated corrosion resistance of all tested alloys was within the range which can be considered as suitable for biodegradable applications. Therefore it is believed that the new alloy at 2% Nd concentration, Mg-5Zn-2Nd-0.13Y-0.35Zr, can be considered as a potential candidate for biodegradable implants. 
 

  • Regenerative Medicine
Location: Paris, France
Speaker
Biography:

Dr. Kavitha Kandiah is working as a UGC-Dr.D.S.Kothari postdoctoral fellow in Actinolaboratry, Microbiology department, Periyar University, Tamil Nadu, Salem, India. Currently, she is working on the development of Nanocomposite materials for diverse field of biomedical application. She has received many laurels and awards such as, Best women researcher award and Best Young Researcher award on 2016 and 2017 respectively. She has credit to 18 research papers in reputed International journals with total 212 Citations and Cumulative IF of 43.242 and attended 20 National and International Conferences

Abstract:

Graphene oxide (GO) reduction, in particularly, the simple, cost effective and environmental benign microbial method attracts high over the other methods. Extracellular mediated actinobacterial reduction of nano graphene oxide is focused for the high purity and productivity of the product without any toxicity. In addition, Indian plant Cynodon dactylon also have an excellent ability to reduce the GO. Reduction of the nano-graphene oxide (nGO) to graphene nanoparticles (nRGO) was identified by visual colour change from brown to black. Further, the reduction of nGO into nRGO was confirmed through XRD pattern. Fig. 1 shows the disappearance of the peak at 2θ = 10° in the GO and formation of new broad peak at 2θ = 26° (JCPDS no. 411487) supports that the GO is completely reduced into graphene. The average Crystallite size of rGO is 8-10nm (Scherrer’s equation).  Further the bioactivity of biological reduced nano graphene give the superior results compare than the chemically synthesized particles. Appropriate degradation as well as HAp layer formation supports the biological synthesis and suggests for the fruitful outcome for therapeutical applications

  • Stem cell therapy
Location: Paris, France

Session Introduction

Lamia Yazgı Alyazici

Yeditepe University,Turkey

Title: Mesenchymal Stem Cell Expansion with Small Molecules
Speaker
Biography:

L. Yazgı Alyazıcı has completed her BSc on Molecular Biology and Genetics at İstanbul University. She is now a master student at Yeditepe University, Biotechnology Department. She is conducting two different projects simultaneously: Mesenchymal Stem Cell Expansion and Pathogen Box Identifiation in Hematopoietic Stem Cells. She also participated in different projets at her lab, including; Cardiogenic Regeneration, Hematopoietic Stem Cell Expansion, Gene Silencing with siRNA. She is currently the laboratory manager at Regenerative Biology lab., supervised by Assoc. Prof. Fatih Kocabaş.

Abstract:

Mesenchymal stem cells are present in the adult body, they can self-renew themselves and exhibit multipotency. They can differentiate to bone, fat, chondrocyte, and other various cell types under specific conditions. They are a great candidate for transplantation-based therapies with their immunomodulatory abilities, differentiation potentials and because of their easy accessibility.  They can be obtained from different tissue types including; bone marrow, adipose tissue, umbilical cord etc. Isolation of MSC is easy but there are major challenges on mobilization, expansion, understanding the differentiation mechanism. If these challenges overcame, MSCs show great potential for experimental and clinical applications. In our study we focused on expansion of mouse bone marrow-MSCs, with small molecule treatment. We selected the effective molecules conducting WST-1 assay, pyronin y/hoechst staining, cell cycle analysis, apoptosis analysis and settled on four different molecules. Our first and most effective molecule is a GSK-3 inhibitor that stabilizes free cytosolic β-catenin and inhibits differentiation. Second one is a p38-MAPK inhibitor. Our goal after this study is to carry this knowledge to therapeutic field

  • Bioengineering
Location: Paris, France

Session Introduction

IMAD M. MOHAMED TAHIR

Duhok University, Iraq

Title: SYNTHESIS OF WATER-SOLUBLE 2-FORMYLBODIPY DYES
Speaker
Biography:

Imad M. MOHAMED TAHIR  was born in Mosel city in 1983. After receiving primary education in Mosel and Karama town, he graduated from secondary school in IRAQ. He received a B.Sc. in chemistry science (2007) from Mosel University. Later, He completed M.A postgraduate programme in organic chemistry at Bingol University, Turkey. He is working in Quality control in Zakho District of the Dohuk Governorate of Iraqi Kurdistan as well as luctrar in Duhok university.

Abstract:

Since 1968, BODIPY dyes have been widely used as fluorescence compounds in many applications due to their high absorption coefficient, fluorescence quantum yield, and good photochemical stability as well as other beneficial properties, such as the ability to be unaffected by the pH and polarity of the environment in which they are placed, which enables them to remain stable for long periods of time. However, BODIPY cores are hydrophobic; thus, they impede labeling in biological situations. Solubility in water is critical if fluorescent probes are to be used in biological conditions. The application of probes in molecular situations is also deterred by quenching or aggressiveness in water. Thus, it is important to develop a new water-soluble BODIPY dye using spectroscopic methods. The study aimed to synthesize a new water-soluble BODIPY dye by introducing quaternary ammonium groups into the BODIPY core. The water-soluble BODIPY dye containing quaternary ammonium groups was created by introducing the water-solubilizing groups onto the meso position followed by formation of the quaternary ammonium groups using methyl iodide or 1,3-propanesultone. new and competent technique was extended and optimized for the successful synthesis of water-soluble BODIPY dyes via introducing hydrophilic groups. Moreover, with the aid of this new methodology, various derivatives of water-soluble BODIPY dyes were synthesized in moderate to good yields under mild reactions conditions. In addition, the proposed dye was verified to be soluble in water. The BODIPY water-soluble dyes present application potentials in cell imaging, labelling of bio-macromolecules, chemosensors, pH indicators, and photodynamic therapy. It is a general feeling that for them to be effective in application their optical properties and biocompatibility need to be enhanced and optimized by robust molecular design. In addition, we believe that our target BODIPY dye can also offer future application potential in the determination generally used to avert foods browning and as antioxidant and antibacterial in beverages (wine).

  • Molecular Biology
Location: Paris, France
Speaker
Biography:

Hana’a Iqbal has completed her PhD in 2014 from Dr. Panjwani Center for Molecular Medicine and Drug Research (PCMD), ICCBS, University of Karachi and currently receiving postdoctoral training from Genetic Engineering and Biotechnology Institute, TÜBÄ°TAK-MAM, Gebze, Kocaeli, Turkey. She has worked as an Assistant Professor previously at SZABIST, Karachi. She has published 6 papers in reputed journals. Currently, she is working on animal model development for bone repair and has future plans to develop cancer model.
 

Abstract:

Advancing cellular age is associated with derailed cell function and repair. Cellular senescence has been implicated strongly in failure of autologous stem cell transplantation. Cellular aging has been linked to several cell intrinsic molecular pathways. The events that are associated with senescence and aging include DNA damage, telomere shortening, accumulation of ROS, increase in cyclin-dependent kinase inhibitor p16INK4, and epigenetic modifications such as DNA methylation. One of these changes, the accrual of reactive oxygen species (ROS) is the foremost factor which accelerates the processes of cellular senescence and cell death. Generation of reactive oxygen species (ROS) and ultimately the oxidative stress is increased during the process of aging. The capacity of self-renewal of stem cells is associated with the control of oxidative stress. Current studies give strength to the hypothesis that cell behavior and consequently tissue regeneration and repair is affected by pretreatment of stem cells with pro- or antioxidants agents. Preconditioning the cells with antioxidants might improve the regeneration potential of these cells and prevent the process of aging in the stem cells.
The present study demonstrates that mesenchymal stem cells (MSCs) particularly oMSCs can be preconditioned with compounds potentially possessing antioxidant activity resulting in the decrease in the ROS content of the cells, thus can improve the regenerative potential of these cells for cell-based therapeutics for various diseases. These compounds can be further developed into drugs to complement the cell-based therapies by increasing the regenerative potential of autologous stem cells via maintenance of cellular oxidative balance.
 

  • Gene Therapy
Location: Paris, France
Speaker
Biography:

Prof. Nguyen Thanh Liem is the Director of Vinmec research institute of Stem cell and gene technology, a leading organization studies regenerative medicine in Vietnam. He is a pioneer and innovator in pediatric endoscopic surgery and stem cell transplatation for unet diseases such as bronchopulmonary dysplasia, autism, liver cirrhosis and cerebral pals in Vietnam. He has published more than 75 studies on the international journal s and has been serving as an editorial board member of some journals. He has been awared the 2018 Nikkei Asia Prize in Science and Technology.

Abstract:

The aim of this study was to evaluate the effectiveness of transplantation of autologous bone marrow mononuclear cells (BMMCs) for improving gross motor function and muscle tone in children with Cerebral Palsy (CP) related to neonatal icterus.This open-label, uncontrolled clinical trial, which included 25 patients with CP related to neonatal icterus who had a Gross Motor Function Classification System (GMFCS) score between level II and level V, was conducted between July 2014 and July 2017 at Vinmec International Hospital (Vietnam). BMMCs were harvested from the patients’ iliac crests. Two BMNMC transplantations via the intrathecal route were performed: the first transplantation was performed at baseline, and the second transplantation was performed 6 months after the first transplantation. Gross motor function was measured at baseline, 6 months, and 12 months using the Gross Motor Function Measure (GMFM) and the Modified Ashworth Scale was used to measure the improvement of muscle spasticity.
 

  • Biomedicine
Location: Paris, France
Biography:

Roni Lara Moya has completed his studies in Biomedicine from the University of Mogi das Cruzes, Sao Paulo. He has obtained his specialization in Anti-Aging Medicine from Seville University, Spain. He completed his Master of Science in Molecular and Cellular Immunology and Biology from the University of Coimbra, Portugal and Master of Science in Clinical Advanced Nutrition from the University of Barcelona, Spain. He completed his PhD in Biomedicine and Immunology from the Gulbenkian Institute of Science and Coimbra University. He is the Coordinator of Orthomolecular Medicine of ReGenera Research Group for Aging Intervention. He is the Professor and Director of the Graduation Program in Orthomolecular Therapy - CESPU University, Portugal. He is the Scientific Advisor for Nutraceuticals and Cell Therapy Companies in Europe.

Abstract:

Metastatic cancer is often a fatal disease with low survival rate that in a course of its progression implies a pathogenic cascade involving inflammation, overexpression of reactive oxygen species, loss of DNA repair, genome instability, neoangiogenesis, epithelial infiltration, collagen destruction, and immunosuppression and apoptosis evasion by cancer cells. Understanding the cross-interaction mechanism between the immune co-stimulatory and inhibitory molecules on one side and tumors cells is a key point in the development of a successful immunotherapeutic strategy to fight aggressive cancers. The active specific immunotherapy (ASI) and the GC protein macrophage activating factor (GcMAF) are two immunotherapies capable of modulating the innate and adaptive immunity against cancer. The aim of this talk is to present a case of an invasive metastatic carcinoma treated with ASI and GcMAF Forte and discusses the potentials of the individualized immunotherapy in advanced forms of cancer. Immunotherapy improves status of immune system and as a result it increases patient’s life span (and probably survival rate). In conclusion, the ASI and GcMAF Forte may offer a promising prospective immunological biomedical approach to boost immunity and enhance life expectancy in patients with metastatic cancer.

 

  • Stem Cell Biology
Location: Paris, France

Session Introduction

Mahdi Lotfipanah

Royan Institute for Reproductive Biomedicine- ACECR, Iran

Title: Stem cell researches on a visual landscape during past five years
Biography:

`Mahdi Lotfipanah is an MSc top student in Medical Library and Information Sciences at Tehran University of Medical Sciences and Librarian of Royan Institute for Stem Cell Biology and Technology for more than 13 years. He is the Protocol Manager of ROYAN Congress and Award and Executive Member in Cell Journal which is an ISI journal in Cell Biology (IF: 2.363). He has some experience and scientific collaboration with epidemiology group of ROYAN Institute. He has published more than 4 papers in reputed journals. In 2016, he got the crystal statue of the best student in the university. He has many papers in the field of Library and Information Sciences in Persian language. He participated in congresses for Illustrated Evaluation of Scientific Productions before.

Abstract:

Nowadays the evaluation, assessment, and analysis of scientific outputs of universities, scientific centers, and scientific publications are considered necessary through bibliometrics and scientometrics. In this regards the evaluation of scientific documents in the field of stem cells in national and international journals indexed in the web of science (ISI) database is no exception. Therefore, publishing articles in high-quality journals with more collaboration with the other scientists in all around the world are highly recommended to authors due to more turnovers and maximizing the effectiveness of scientific production. This paper studied journal of “Cell” outputs as a sample of high-quality and efficiency scientific journal in the field of stem cells during 2013 till the end of 2017 to show the trends of stem cell researches, to investigate some network and links of “academic communications”. This study is expected to give insights of how distinguished researchers in the field of stem cells thought during past five years and may unearth some absorbing features and landscape by constructing and displaying large bibliometric maps and graphical representation.
 In this research, data and outputs of the journal of Cell were collected from WoS database using the scientometric methods. Data visualization was done by using VOSviewer software which is a computer program for creating maps based directly on the network, bibliographic data and data text which can visualize and explore these maps. The present evaluation method is called scientometrics which is used by researchers in the field of future studies or information sciences. A total of 3102 documents were extracted from the web of science database and analyzed. Original articles, review papers and editorial materials were considered for analyzing. This publication was identified with the keywords: “Cell” in publication name item and we chose 2013 till 2017 for analyzation.