Matej Buzgo
Inocure s.r.o , Czech Republic
Title: Cell-free tissue engineering based on drug delivery enhancement of regeneration
Biography
Biography: Matej Buzgo
Abstract
Cell based tissue engineering suffers from wide range of ethical, legal and technical obstacles, which limit clinical use of developed tissue. An alternative approach proposed in present work is based on utilization of scaffolds with embedded drug delivery systems as cell-free systems. We have successfully developed novel drug releasing scaffolds based on core/shell nanofibers prepared by electrospinning and centrifugal spinning technology. The technology enables long term preservation and release of protein based active molecules. We have utilized the technology for delivery of synthetic growth factors (i.e. TGF-beta, bFGF, IGF-I) and natural growth factors (i.e. platelet rich plasma). Scaffolds enabled long term delivery of active molecules in order of 3-4 weeks with regulation of release half-life by changing of core/shell ratio and crosslinking rate. In order to demonstrate feasibility of technology. We have showed bioactivity both in vitro and in vivo. The scaffolds were shown to improve bone and cartilage healing of both small and large animal models. The formed tissue showed homogenous distribution and quality bone tissue formation.
The results of studies demonstrate potential of cell-free scaffolds as an viable alternative of classical route of tissue engineering. Such alternative approach reduces market barriers leading to efficient ATMPs uptake by market and fosters application of tissue-stimulating biopharmaceutical use in clinical practice.