Jeong Ok Lim
Kyungpook National University, South Korea.
Title: Fabrication of 3D printed dressing composed of VEGF peptide and hydrogel complex and evaluation for its wound healing capacity in vitro and in vivo
Biography
Biography: Jeong Ok Lim
Abstract
Printing technology offers a possibility of printing structurally complicated tissue scaffolds. Currently hardware and software of bioprinters are commercially available and printing three dimensional tissue contracts becomes possible. However, hurdles we face when we print biological structure are lacking of bioinks of good quality. Therefore, it is demanding to develop bioinks to successfully print biological matrix. Hence our research objective is to develop and optimize a bioink. We formualted a new bioink and investigated its rheological, chemical and biological properties for applicability to print skin patch for wound healing. This bioink is composed of VEGF peptide with 15 amino acids and hydrogel complex.. The peptide sequence, hydrogel-peptide ratio, stability and release kinetics were investigated for optimal cell viability, angiogenesis, skin regeneration and ultimately wound healing. Formulation was characterized in vitro for its 3D printability, resolution, temperature condition, mechanical stability, cell biocompatibility and degradability. And skin patch or dressing was examined for its safety and efficacy of wound healing in vivo using rat and pig model. The results from experiments demonstrated that newly formulated bioink is easy to print, gelling, not toxic, stable, and low cost. Therefore, VEGF peptide-gel complex has a great potential as a bioinlk for printing three-diemnsional structure for tissue reconstruction.