Scientific Program

Conference Series Ltd invites all the participants across the globe to attend 13th Annual Conference on Stem Cell and Biomaterials Nice, France | Park Inn Nice Airport Hotel 24 Rue Costes et Bellonte, 06200 Nice, France.

Day 1 :

Keynote Forum

Jacqueline Jacques

BioQuantique SARL, Switzerland

Keynote: Stimulate the patient’s OWN Stem Cells with the Quantum Technologies SCIO / EDUCTOR

Time : 10:30-11:45

Conference Series Euro Biomaterials 2019 International Conference Keynote Speaker Jacqueline Jacques photo
Biography:

Jacqueline has more than 30 years of experience as a Manager, Management Consultant, and Coach mainly in Canada, USA, France Hong Kong and UK. She trained from experts and teachers in Personal Development and Alternative Medicine in Europe, America and Asia becoming a Doctor of Sciences specializing in Quantum Energy Medicine. She is a Lecturer, Seminar leader, Coach and Quantum Biofeedback & Bioresonance Expert promoting Quantum Technologies & Therapies and training & coaching practitioners in their understanding and practice of this new approach to health and wellness. She is the Founder and Chairperson of BioQuantique SARL (Geneva) and BioQuantum (Canada).

 

Abstract:

Many researchers believe that "Over the next two decades, stem cells will change medicine forever, extend life and, potentially, save our life!" Yes, stem cells have tremendous promise to help us understand and treat a range of diseases, injuries and other health-related conditions. However, very few stem cell treatments have been proven safe and effective.

By stimulating the capacity of the patient to produce his/her OWN stem cells, Quantum Technologies SCIO/EDUCTOR, based on Quantum Physics, Biofeedback and Bioresonance provide a safe and effective alternative to stem cells complexes & costly medicalized treatments & procedures that still need to be validated.

The main premises of Quantum Biofeedback & Bioresonance therapies are:

 

  • The energetic body is the matrix of the physical body. Stimulate and heal the energetic body and the biochemistry of the body will change.

 

  • The body is innately intelligent and it has the ability to heal itself if the right conditions or stimuli (frequencies) are provided.

 

  • Electromagnetic frequencies (micro-currents) can be used to re-establish the electrical and biochemical functions of the body to restore the integrity of the tissue and promote healing

 

We are beyond just chemistry! Life is Photonic! Cells communicate with electro-magnetic radiation. We can use electro-magnetic radiation to modulate stem cells and stabilize the energy in the body. The SCIO/EDUCTOR Quantum Technology integrate + 500 focused energetic therapies including balancing and stimulating stem cells, stimulating cell communication, regeneration therapies, DNA harmonization etc. Stem cells as well as the organs producing stem cells, (bone marrow, adipose tissue, etc.) can be stimulated and modulated through electromagnetic radiation (micro-currents).The SCIO/EDUCTOR Quantum Technologies & Therapies work with photons, electrons and electromagnetism (micro-currents) to improve the electrical vitality of the body’s cells and organs and to re-establish homeostasis in the body. Quantum Technologies & Therapies thus are the most appropriate therapies when considering stem cells!

Join us for this presentation where we will demonstrate some of the most powerful functionalities of SCIO/EDUCTOR Technology for stimulating our patient’s capacity to produce their OWN Stem Cells through a safe, efficient, painless, drug-free, without side-effects and natural process.

 

Keynote Forum

Matej Buzgo

Inocure s.r.o , Czech Republic

Keynote: Cell-free tissue engineering based on drug delivery enhancement of regeneration

Time : 12:15-13:00

Conference Series Euro Biomaterials 2019 International Conference Keynote Speaker Matej Buzgo photo
Biography:

Matej Buzgo, MSc has experience in development of drug delivery systems by electrospinning, centrifugal spinning and electrospraying. His main interest is in preparation of core/shell nanofibers by needleless electrospinning and centrifugal spinning for drug delivery of proteins and small molecules. He is coauthor of >25 scientific publications, >5 national and European patents and leads one European and one nation grant projects.

 

Abstract:

Cell based tissue engineering suffers from wide range of ethical, legal and technical obstacles, which limit clinical use of developed tissue. An alternative approach proposed in present work is based on utilization of scaffolds with embedded drug delivery systems as cell-free systems. We have successfully developed novel drug releasing scaffolds based on core/shell nanofibers prepared by electrospinning and centrifugal spinning technology. The technology enables long term preservation and release of protein based active molecules. We have utilized the technology for delivery of synthetic growth factors (i.e. TGF-beta, bFGF, IGF-I) and natural growth factors (i.e. platelet rich plasma). Scaffolds enabled long term delivery of active molecules in order of 3-4 weeks with regulation of release half-life by changing of core/shell ratio and crosslinking rate. In order to demonstrate feasibility of technology. We have showed bioactivity both in vitro and in vivo. The scaffolds were shown to improve bone and cartilage healing of both small and large animal models. The formed tissue showed homogenous distribution and quality bone tissue formation.

The results of studies demonstrate potential of cell-free scaffolds as an viable alternative of classical route of tissue engineering. Such alternative approach reduces market barriers leading to efficient ATMPs uptake by market and fosters application of tissue-stimulating biopharmaceutical use in clinical practice.

 

Keynote Forum

Helen Waller

Newcastle University, UK

Keynote: Engineered Caf1 protein polymers form tuneable bioactive hydrogel scaffolds

Time : 13:30-14:00

Conference Series Euro Biomaterials 2019 International Conference Keynote Speaker Helen Waller photo
Biography:

Helen Waller has completed her PhD at Newcastle University in 2007, and she is currently working there as a Postdoctoral Candidate in the Laboratory of Professor Jeremy Lakey. Her current focus is the Development of the Caf1 Protein Polymer for Potential Applications in Tissue Culture and Wound Healing. This is funded via a BBSRC-supported Industrial Biotechnology Catalyst award: Manufacture of Complex Protein Polymers for Industry and Medicine, on which she is one of four Postdoctoral Researchers. Her major role involves the polymer expression and purification with scale up to a bioprocessing level using 2 and 10 L fermenters.

 

Abstract:

Here, we describe a new animal-free biomaterial with potential uses in 3D-tissue culture and regenerative medicine due to its low cost, high stability and definable bioactivity. Capsular antigen fraction1 (Caf1) is a protein from the plague bacterium Yersinia pestis that is secreted via the chaperone-usher pathway and protects the pathogen from phagocytosis by forming a non-stick protective layer around the cell. The 15.5 kDa monomer has an Ig-like fold and resembles the extracellular matrix protein fibronectin. The subunits polymerize via donor-strand complementation, forming a highly stable non-covalent polymer. In this work, recombinant Caf1 polymers produced via batch fermentation using Escherichia coli were secreted by the bacterium into a flocculent layer above the cell pellet, and could be easily extracted and purified in large quantities. We demonstrate the polymers robust thermostability by circular dichroism and SDS-PAGE, and observe their large size using electron microscopy and SEC-MALS. Additionally, we have selectively reversed the natural non-stick behaviour of the WT polymer by introducing an integrin binding sequence, RGDS, into loop 5 that can promote U2OS cell adhesion. Additional bioactive peptides motifs from Osteopontin, bone morphogenic protein 2, collagen and laminin were then introduced at different positions within Caf1. Finally, PEG based chemical cross linkers were used to form stable 3D hydrogels with designed porosities and tuneable stiffness, ideal for use in cell culture and drug delivery applications. The combination of these new motifs into tuneable Caf1 hydrogels will help to expand the functionality of this exciting new biomaterial for use in a variety of biomedical applications.

 

Keynote Forum

Siddharth Pandey

Datt Media Products Pvt.Ltd, India

Keynote: Role of biodegradable polymers in bio-medical application and regenerative medicine

Time : 14:00-14:30

Conference Series Euro Biomaterials 2019 International Conference Keynote Speaker Siddharth Pandey photo
Biography:

Siddharth Pandey has completed his PhD in Biotechnology, specialized in Stem Cell Biology and Tissue Engineering from Institute of Nuclear Medicine and Allied Sciences (INMAS), a unit of DRDO and Jamia Hamdard. He has started his carrier at Datt Mediproducts Pvt. Ltd., and currently working as the Manager and Head of Department of Life Science at Datt Mediproducts Pvt. Ltd. In his thesis work, he focused on Stem Cell Microencapsulation. His research interests lie in the area of stem cell biology and tissue engineering. Apart from research, he also has very sound knowledge in regulatory affairs, clinical research, and technology transfer and product commercialization. He has three international publications and filled more than 15 patents and four US patents were granted.

 

Abstract:

Utilization of polymers as biomaterials has greatly impacted the advancement of modern medicine. In recent years, a wide range of natural and synthetic degradable polymers have been investigated for tissue engineering applications with novel materials constantly being developed to meet new challenges. Hemostatic products are widely used to accelerate blood clotting time in case of surgical or traumatic wounds. Severe bleeding from the wound results into loss of blood which in turn may lead to hypovolemic shock affecting tissue and organ damage. In order to stop the bleeding of wounds several active ingredients like thrombin, gelatin, collagen, fibrin, etc. are used for manufacturing hemostatic products. Our invention provides a ready to use biodegradable and biocompatible device based on chitosan and gelatin polyelectrolyte complex (PEC). The PEC is a novel porous scaffold which can be used as carrier to stop the flow of blood under surgical conditions and in other biomedical applications. The PEC scaffold is mainly impregnated with various clotting factors and clot stabilizers like thrombin, calcium chloride, glucosamine and tranexamic acid. The scaffold can be directly applied to the wound site in order to stop the bleeding with immediate results. The PEC scaffold shows very good biocompatibility, biodegradability, low toxicity and low immunogenicity at the site of injury. The preclinical studies and clinical studies data confirmed the efficacy of this product.

 

Keynote Forum

Ruijiao Dong

Imperial College London, UK

Keynote: Synthesis and application of precision biopolymers with perfectly defined monomer sequence

Time : 14:30-15:00

Conference Series Euro Biomaterials 2019 International Conference Keynote Speaker Ruijiao Dong photo
Biography:

Ruijiao Dong has received his BSc degree in Materials Science from Wuhan Textile University in China and MSc degree in Polymer Chemistry from Donghua University, China. In 2014, he has completed his PhD in Polymer Chemistry at Shanghai Jiao Tong University in China. Currently, he joined the Livingston Group at Imperial College in UK as a Research Associate. His research interests focus on the Design, Synthesis and Application of Functional Supramolecular Polymers, Precision Polymers And Sequence-Defined Polymers. He has published more than 23 papers in reputed peer-reviewed journals, including Nature Chemistry, Advanced Materials, Angewandte Chemie, Accounts of Chemical Research, ChemComm, ACS Applied Materials & Interfaces, etc.

 

Abstract:

Nucleic acids and proteins are exactly sequence-defined biomacromolecules who’s specifically ordered sequences of nucleotide or amino acid monomers create complexity and assure the structural and functional diversity required for living organisms. Monomer sequence control is the key strategy used by nature for developing molecularly encoded self-replicators, which are the essence of all of Earth’s known life forms. However, current synthetic polymers are often polydisperse and sequence-uncontrolled in nature, which greatly hinder understanding of fundamental interactions between synthetic polymers and biological systems. In recent years, various synthesis strategies have been developed to fabricate precision synthetic polymers. For example, solid-phase chemistry allows preparation of sequence-defined polymers. Such strategy is quite efficient for controlling dispersity and monomer sequences of synthetic polymers, but it is greatly limited by low yields, high cost of the solid supports, inability to quantitatively monitor the coupling progress and difficulties of scale up. In striking contrast, liquid-phase synthesis has long been proposed to overcome these deficiencies, enabling facile and efficient production of precision synthetic polymers at large scales. Herein, we create a flexible and scalable platform towards manufacturing various precision biopolymers (i.e. polyether’s, polyesters, etc.) with readily controlled side-chain sequences. In this strategy, a discrete polymer backbone with defined monomer sequences will be readily fabricated using liquid-phase iterative synthesis with size-exclusion molecular-sieving followed by site-selective conjugation of versatile functional theranostic agents to the polymer backbone in a specific order, to produce multifunctional precision biopolymers. The resulting multifunctional precision biopolymers have great potential as a promising theranostic vehicle for widespread applications in biomedical and pharmaceutical fields.

 

Conference Series Euro Biomaterials 2019 International Conference Keynote Speaker Fazliny Abd. Rahman photo
Biography:

Periodontal ligament (PDL) contains a unique population of mesenchymal stem cells (MSCs), also known as PDL stem cells (PDLSCs). The regenerative properties of PDLSCs hold great potential for its use in stem cells based therapy, particularly for periodontal or bone regeneration. There have not been many studies examining the effect of ASA on stem cells, notably PDLSCs. The present study investigated the global gene expression profile in PDLSCs during osteogenic differentiation. MSCs from PDL were isolated from normal permanent human teeth (n=3). Microarray analysis was used to study the effects of ASA (200, 500, and 1,000 μM) on the gene expression profiles in PDLSCs during osteogenic differentiation. Microarray study revealed that ASA was able to modulate PDLSCs gene expression profile. At 200 µM, 315 genes were DE, involving 151 upregulated and 164 downregulated genes. At 500 µM, 794 genes were DE, involving of 364 upregulated and 430 downregulated genes. At 1000 µM, the number of DE genes increased to 2035, of which 735 were upregulated and 1300 were downregulated. Bioinformatics analyses of the gene expression data revealed that the majority of DE genes (for 500 and 1,000 µM ASA treatment) are involved in osteogenic differentiation. The gene network analysis was carried out using Ingenuity Pathway Analysis (IPA) software, and this revealed that the number of gene groups involved in cell adhesion and extracellular matrix components were increased. This study indicated that ASA could enhance PDLSCs functions and provide evidence for the potential use of ASA with PDLSCs for regenerative dentistry applications, particularly in the areas of periodontal health and regeneration.

 

Abstract:

Fazliny Abd. Rahman has completed her PhD from the University of Malaya. She is currently teaching Biochemistry for Dental students at Faculty of Dentistry, SEGi University, Kota Damansara, Selangor, Malaysia.

 

Keynote Forum

Orly Hakimi

Shamoon College of Engineering , Israel

Keynote: A study on mechanical properties and corrosion resistance of Mg-Zn-Nd alloys as potential biodegradable implants

Time : 15: 30-16:00

Conference Series Euro Biomaterials 2019 International Conference Keynote Speaker Orly Hakimi photo
Biography:

Mg alloys are considered as potential structural materials for biodegradable implants mainly due to their excellent biocompatibility, degradation behavior in in-vivo conditions and adequate mechanical properties. However, their accelerated corrosion rate in physiological environments may lead to premature loss of mechanical integrity and cytotoxic effects. Here we characterized the corrosion behavior and mechanical properties of a novel magnesium-zinc alloy, Mg-5%Zn-0.13%Y-0.35%Zr with up to 3% Nd additions following a homogenizing treatment and extrusion process, with regards to serving as a biodegradable implant. The microstructural characteristics were examined by optical microscopy, scanning electron microscopy (SEM) and X-ray diffraction analysis. The corrosion performance examination was carried out under in-vitro conditions, including immersion testing, electrochemical analysis, and stress corrosion cracking (SCC) assessments in terms of slow strain rate testing (SSRT), all in PBS solution. The mechanical evaluations included hardness and tensile examinations. The obtained results clearly demonstrated an optimal combination of strength and ductility for the new alloy at 2% Nd concentration. This was attributed to an optimal concentration of the secondary phase, W-phase (Mg3(Nd,Y)2Zn3), generated at grain boundaries. The addition of different concentrations of Nd to the base alloy, resulted in minor effect on the corrosion resistance, nevertheless, the calculated corrosion resistance of all tested alloys was within the range which can be considered as suitable for biodegradable applications. Therefore, it is believed that the new alloy at 2% Nd concentration, Mg-5Zn-2Nd-0.13Y-0.35Zr, can be considered as a potential candidate for biodegradable implants.

 

Abstract:

O Hakimi has completed her PhD in Material Engineering from Ben Gurion University of the Negev, Israel, in August 2016. She is a Lecturer in the Department of Mechanical Engineering at SCE-Shamoon College of Engineering, Israel. In addition, she is an Academic Coordinator of Manufacturing Technologies and Material Science courses and laboratories. She is a Specialist in biodegradable magnesium implants, processing, including additive manufacturing, and physical metallurgy of alloys and environmental behavior of metals. She has published six papers in reputed journals and has been serving as a Reviewer for MDPI Journals.

 

Keynote Forum

Annett Dorner-Reisel

University of Applied Sciences Schmalkalden, Germany

Keynote: Wear resistant diamond-like carbon coatings on femoral parts of knee prostheses

Time : 16:00-16:30

Conference Series Euro Biomaterials 2019 International Conference Keynote Speaker Annett Dorner-Reisel photo
Biography:

CoCrMo-alloys form a thin oxide film, primarily of Cr2O3 with small amounts of Co3O4 and MoOx with a reported thickness of about 2 nm. The thin oxide film improves the biocompatibility. However, the oxide film locally damages due to tribological or tribo-chemical impacts, i.e. the harsh conditions in a load carrying artificial knee joint. Both tribological pairs of these artificial joints, the CoCrMo metallic femur and the ultrahigh molecular weight polyethylene (UHMWPE) inlay show wear debris in clinical observations. Such particulate wear debris and metallic ions generated from the worn surfaces of CoCrMo and UHMWPE can pose a severe threat to human tissues, which leads to failure of implants and/or the need for revision surgeries. It is known, that metallic corrosion and wear products exist as protein bone complexes which had the ability to induce bone resorption in vitro. High concentration of Co2+ ions in tissue are correlated to inflammation in vitro. Such negative and toxic impacts of metallic ions and particles on tissue are called metallosis. Different wear resistant and bio-inert or antibacterial (i.e. Ag-doped) DLC-coating types are investigated over decades as surface toping on loaded implants. DLC with tailored nanostructure on CoCrMo femoral part prevents metallosis of these alloys. The contribution discusses results from testing DLC coated femoral parts 5 million cycles in a knee joint simulator according to the standard ISO/DIS 14243. Raman spectroscopy confirms a similar structure of DLC coating before and after testing according to ISO/DIS 14243. The DLC remained unworn and structural similar due to the wear tests. However, different coating thickness resulted in different material loss from the UHMW-PE inlays. The reason may be, heat generation in the tribological micro-contacts on UHMW-PE surface, if DLC coating thickness increased. In order to improve the heat transfer and wear behaviour, we suggest a laser treatment and generation of biomimetic surface structures. 

Abstract:

After a training as technician for ceramic processing in 1989 and studies in process and materials engineering, Annett Dorner-Reisel, worked as scientific staff. She received her PhD at the age of 27 years from TU Chemnitz (D). She specialized in biomaterials and hybrid structures for biomedical and functional materials for transportation and energy sector, i.e. sensor/actor applications or high performance carbon allotropes. Dr. Dorner-Reisel carried out postdoctoral studies at UC Santa Barbara, USA and Nagoya Institute of Research, Japanmailto:[email protected] Following second doctoral degree (Habilitation) at the age of 32 years at the Bergakademie Freiberg (D), she worked several years in industry before engaging 2011 in a professorship position at the Schmalkalden University HS SM. Since 2017, Prof. Dr.-Ing. habil. Dorner-Reisel is head of the research group “Bio-STEP” at HS SM, which clusters several researchers from different working directions like materials, production technology electronics and signal transfer. Bio-STEP focuses on biomaterials, biogenic materials for energy and light weight sectors, bionics and biomimetic.